REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-NeuN Antibody, clone A60	Sigma-Aldrich	RRID: AB_2298772
Donkey anti-Mouse IgG Secondary Antibody	Thermo-Fisher	RRID: AB_2762830
Bacterial and virus strains
AAV1-EF1a-doublefloxed-hChR2(H134R)-EYFP-WPRE-HGHpA	unpublished	RRID: Addgene_20298
AAV1-hSyn1-SIO-stGtACR2-FusionRed	Mahn et al., 2018	RRID: Addgene_105677
AAV1-Syn-Flex-GCaMP6f-WPRE-SV40	Chen et al., 2013	RRID: Addgene_100833
AAV1-hSyn-DIO-hM4D(Gi)-mCherry	Krashes et al., 2011	RRID: Addgene_44362
AAV1-EF1a-FLEX-EGFP	SWC Viral Vector Core	N/A
AAV1-EF1a-FLEX-mCherry	SWC Viral Vector Core	N/A
AAVretro-hSyn-Cre	SWC Viral Vector Core	N/A
Chemicals, peptides, and recombinant proteins
DiI	Thermo-Fisher	Cat# D3911
CNO	Sigma Aldrich	Cat# SML2304
Picrotoxin	Bio-Techne	Cat# 1128
Kynurenic acid	Sigma Aldrich	Cat# K3375
Experimental models: Organisms/strains
Slc32a1tm2(cre)Lowl	The Jackson Laboratory	RRID: IMSR_JAX:028862
C57BL/6J	Charles River	RRID: IMSR_CRL:27
Ai14D	The Jackson Laboratory	RRID: IMSR_JAX:007914
Software and algorithms
MATLAB R2017a	The MathWorks Inc.	https://uk.mathworks.com/products/matlab.html; RRID: SCR_001622
Psychophysics Toolbox Version 3	Brainard, 1997; Pelli, 1997; Kleiner et al., 2007	http://psychtoolbox.org; RRID: SCR_002881
ScanImage	Pologruto et al., 2003	Vidrio Technologies, LLC; RRID: SCR_014307
Kilosort2	Pachitariu et al., 2016	https://github.com/MouseLand/Kilosort; RRID: SCR_016422
Phy	Rossant et al., 2016	https://github.com/cortex-lab/phy
DeepLabCut	Mathis et al., 2018	https://github.com/DeepLabCut/DeepLabCut
SpikeGLX	Janelia Research Campus	https://github.com/billkarsh/SpikeGLX
PyPhotometry	Akam and Walton, 2019	https://pyphotometry.readthedocs.io/en/latest/
Mantis	TANDM Solutions Limited	https://www.mantis64.com/