| Antibody |
CycT1 (E-3) (mouse monoclonal) |
Santa Cruz Biotechnology |
sc-271348 |
WB (1:200) |
| Antibody |
CDK9 (F-6) (mouse monoclonal) |
Santa Cruz Biotechnology |
sc-376646 |
WB (1:200);IP 4 μg per test |
| Antibody |
PKCα (H-7) (mouse monoclonal) |
Santa Cruz Biotechnology |
sc-8393 |
WB (1:200) |
| Antibody |
PKCβ1 (E-3) (mouse monoclonal) |
Santa Cruz Biotechnology |
sc-8049 |
WB (1:200) |
| Antibody |
PKCβ2 (F-7) (mouse monoclonal) |
Santa Cruz Biotechnology |
sc-13149 |
WB (1:200) |
| Antibody |
PKCε (E-5) (mouse monoclonal) |
Santa Cruz Biotechnology |
sc-1681 |
WB (1:200) |
| Antibody |
CDK9 (EPR22956-37) (rabbit polyclonal) |
Abcam |
ab239364 |
WB (1:2000);IP 4 μg per test |
| Antibody |
HA (clone HA-7) (mouse monoclonal) |
Sigma-Aldrich |
H3663 |
WB (1:1000);IP 4 μg per test |
| Antibody |
Flag (clone M2) (mouse monoclonal) |
Sigma-Aldrich |
F1804 |
WB (1:1000);IP 4 μg per test |
| Antibody |
HA (rabbit polyclonal) |
Sigma-Aldrich |
H6908 |
WB (1:1000);IP 4 μg per test |
| Antibody |
Flag (rabbit polyclonal) |
Sigma-Aldrich |
F7425 |
WB (1:1000);IP 4 μg per test |
| Antibody |
CycT1 (D1B6G) (rabbit monoclonal) |
Cell Signaling Technology |
81464S |
WB (1:1000) |
| Antibody |
β-Actin (13E5) (rabbit monoclonal) |
Cell Signaling Technology |
4970S |
WB (1:5000) |
| Antibody |
Phospho-threonine (42H4) (mouse monoclonal) |
Cell Signaling Technology |
9386S |
WB (1:500) |
| Antibody |
Phosphoserine (rabbit polyclonal) |
Abcam |
ab9332 |
WB (1:500) |
| Antibody |
Phospho-tyrosine (P-Tyr-100) (mouse monoclonal) |
Cell Signaling Technology |
9411S |
WB (1:500) |
| Antibody |
Normal rabbit control IgG |
Santa Cruz Biotechnology |
sc-2027 |
IP 4 μg per test |
| Antibody |
Normal mouse control IgG |
Santa Cruz Biotechnology |
sc-2050 |
IP 4 μg per test |
| Antibody |
Amersham ECL Mouse IgG, HRP-linked whole Ab (from sheep) |
Cytiva |
NA931 |
WB (1:10,000) |
| Antibody |
Amersham ECL Rabbit IgG, HRP-linked whole Ab (from donkey) |
Cytiva |
NA934 |
WB (1:10,000) |
| Chemical compound, drug |
Bortezomib |
Calbiochem |
179324-69-7 |
|
| Chemical compound, drug |
Okadaic acid |
Cell Signaling Technology |
5934S |
|
| Chemical compound, drug |
Staurosporine |
Selleckchem |
S1421 |
|
| Chemical compound, drug |
Sotrastaurin |
Selleckchem |
S2791 |
|
| Chemical compound, drug |
Bisindolylmaleimide IX |
Selleckchem |
S7207 |
|
| Chemical compound, drug |
HBDDE |
Selleckchem |
ab141573 |
|
| Chemical compound, drug |
H-7 |
Abcam |
ab142308 |
|
| Chemical compound, drug |
Cycloheximide (CHX) |
Sigma-Aldrich |
C4859 |
|
| Chemical compound, drug |
Bisindolylmaleimide IV |
Selleckchem |
S0754 |
|
| Chemical compound, drug |
Phorbol 12-myristate 13-acetate (PMA) |
Sigma-Aldrich |
P8139 |
|
| Chemical compound, drug |
Ionomycin |
Sigma-Aldrich |
I9657 |
|
| Chemical compound, drug |
MEK 1/2 inhibitor (MEK 1/2i) |
Calbiochem |
444967 |
|
| Chemical compound, drug |
Enzastaurin |
Selleckchem |
S1055 |
|
| Chemical compound, drug |
VTX-27 |
Selleckchem |
S0069 |
|
| Chemical compound, drug |
Calyculin A |
Cell Signaling Technology |
9902S |
|
| Peptide, recombinant protein |
BSA |
Invitrogen |
AM2616 |
|
| Peptide, recombinant protein |
β-Casein |
Sigma-Aldrich |
C6905 |
|
| Peptide, recombinant protein |
Recombinant human PKCα protein (active) |
Abcam |
ab55672 |
|
| Peptide, recombinant protein |
Recombinant human PKCβ1 protein (active) |
Abcam |
ab60840 |
|
