Fig. 1.

Extent of siRNA encapsulation in LNPs prepared at different mixing speeds determined by the RiboGreen assay. The extent of siRNA encapsulation in LNPs (± PEG-DMG) was calculated using the following equation: $\frac{\left(A,m,o,u,n,t,,o,f,,t,o,t,a,l,,s,i,R,N,A,,i,n,,t,h,e,,s,a,m,p,l,e,-,A,m,o,u,n,t,,o,f,,f,r,e,e,,s,i,R,N,A,,i,n,,t,h,e,,s,a,m,p,l,e\right)}{AmountoftotalsiRNAinthesample}\times 100\%$. SiRNA-LNPs were initially prepared in 10 mM citrate buffer and further diluted to a final siRNA concentration of 50 nM using 1 × PBS. Data are presented as mean ± SD of n = 3 samples. Statistical comparisons were made using two-way ANOVA. *p < 0.05 and ns, non-significant