Fig. 2.

Colloidal stability of blank or siGFP-loaded LNPs measured using dynamic light scattering. SiGFP-LNPs were initially prepared in 10 mM citrate buffer and further diluted to a final siRNA concentration of 50 nM using 1 × PBS (pH 7.4). The samples were stored at 2–8 °C while not in use. Z-average particle diameters (a), dispersity indices (b), and zeta potentials (c) were measured on a Malvern Zetasizer Pro. Data are presented as mean ± SD of n = 3 measurements. Statistical comparisons were made using one-way ANOVA or one-sample t and Wilcoxon tests. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001