Fig. 3.

Colloidal stability of blank- or siGFP-loaded LNPs immediately after preparation (0 h) and upon storage at 37 °C for 24 h measured using dynamic light scattering. SiGFP-LNPs were initially prepared in 10 mM citrate buffer and further diluted to a final siRNA concentration of 50 nM using 1 × PBS at a final pH of 7.4 for particle size and PDI measurements and using deionized water for zeta potential measurements. The samples were stored at 37 °C for 24 h. Z-average particle diameters (a), polydispersity indices (b), and zeta potentials (c) were measured on a Malvern Zetasizer Pro. Data are presented as mean ± SD of n = 3 measurements. Statistical significance was determined using two-way ANOVA Tukey’s and Šídák’s multiple comparisons tests. *p < 0.05, ***p < 0.001, ****p < 0.0001 and ns, non-significant