Fig. 6.

C12-200 LNPs are cytocompatible with TRPV1-expressing breast cancer cell line panel (a), rat primary DRG neurons (b), and MDA-MB-231 cells (c). Cells were incubated with LNPs containing 50 nM siGAPDH (a), 50 nM siTRPV1 (b), and the indicated concentrations of siGFP-LNPs (c) for 4 h. Untreated cells and cells treated with PEI (50 μg/mL) were used as negative and positive controls, respectively. Cell viability was determined 24 h post-transfection using a CellTiter-Glo luminescence viability assay, and the data were normalized to untreated cells. Data represents mean + SD (n = 6)