Table 1.
Primer sequence for detection of gene expression by RT-qPCR.
| Gene | Forward primera | Reverse primera |
|---|---|---|
| Gapdhb | GCCCCTTCTGCCGATGC | CTTTCCAGAGGGGCCATCC |
| Mmp2 | CACCTGGTTTCACCCTTTCT | GAGGAGGGGAACCATCACTA |
| Mmp3 | TGGAGATGCTCACTTTGACGA | CCTTGGCTGAGTGGTAGAGTC |
| Mmp8 | TGGCATTCAGACAATCTATGGACCT | CACGGAGTGTGGTAGTAGCATCAA |
| Mmp12 | GGAGCTCACGGAGACTTCAACT | CCTTGAATACCAGGTCCAGGATA |
| Mmp14 | TCGGCCCTCTGTCCCAGATAA | GCCAGAACCATCGCTCCTTGA |
| Timp1 | CCAGAGCCGTCACTTTGCTT | AGGAAAAGTAGACAGTGTTCAGGCTT |
| Timp2 | TTGCAGGAAAGGCAGAAGGAGATG | GCACTCACAGCCCATCTGGTA |
| Timp3 | CTGACAGGGCGCGTGTATGAA | CAACCCAGGTGGTAGCGGTAATTG |
| Timp4 | AGGGTTCGAGAAGGCCAAGGA | CCATCACTGAGAATCTGGCCAGTCA |
| Park7 | AACACACCCACTGGCTAAGG | CTCCACAATGGCTAGTGCAA |
| RelA | CCTTCCTCAGCCATGGTACCTCT | CCCAAGTCTTCATCAGCATCAAACTGC |
| Nfkb2 | CCCTACCCATCCCTCTCC | CCCGCCTAAGGTGAAGAGA |
| Nrf2 | GATCCGAGATATACGCAGGAGAGGTAAGA | GCTCGACAATGTTCTCCAGCTTCC |
| Hmox1 | GCCCCACCAAGTTCAAACAGCTCTA | CTCTGTCAGCATCACCTGCAGC |
a
Primer sequences given in 5′ to 3′ direction. All primers purchased from IDT, USA. Mmp2, Mmp9 and Park7 primers were purchased from RealTimePrimers.com, USA.
b
GAPDH, glyceraldehyde-3-phosphate dehydrogenase.