FIG. 2.

Suppression of S. cerevisiae sgv1 mutants by C. albicans Crk1. (A) Pheromone-induced growth arrest assay. Haploid S. cerevisiae sgv1 mutants were transformed with vector (pVTU) (1), SGV1 (pVTUSGV1) (2), SGV1N (pVTUSGV1N) (3), CRK1 (pVTUCRK1) (4), and CRK1N (pVYUCRK1N) (5). Approximately 10⁷ cells were plated on each YPD plate. Sterile filter disks were placed on the nascent cell lawns; α-factor in the amounts of 0 ng (top), 50 ng (left), and 500 ng (right) was added to the disks. Plates were incubated for 2 days at 30°C. (B) Effect of temperature on growth. The strains used for panel B were used to test growth properties at 37 and 18°C. Cells were streaked onto SC−Ura plates, which were incubated for 5 days at 30°C, 7 days at 37°C, and 7 days at 18°C.