Fig. 52.

Phylogram generated from maximum likelihood analysis based on combined LSU, SSU, ITS and TEF1-α sequence data representing suborder Massarineae in Pleosporales. Related sequences are taken from Hyde et al. (2020b) and additions according to the BLAST searches in NCBI. Fifty-eight strains are included in the combined analyses which comprised 3070 characters (798 characters for LSU, 961 characters for SSU, 469 characters for ITS, 842 characters for TEF1-α) after alignment. Alternaria alternata (CBS 916.96) and Leptosphaeria doliolum (CBS 505.75) in Pleosporaceae and Leptosphaeriaceae respectively (Pleosporales) were used as the outgroup taxa. The best scoring RAxML tree with a final likelihood value of − 18300.642497 is presented. The matrix had 1007 distinct alignment patterns, with 36.82% of undetermined characters or gaps. Estimated base frequencies were as follows: A = 0.240587, C = 0.242773, G = 0.272823, T = 0.243817; substitution rates: AC = 1.614921, AG = 3.258874, AT = 1.951323, CG = 1.249772, CT = 8.508461, GT = 1.000000; gamma distribution shape parameter α = 0.183977. Bootstrap support values for ML equal to or greater than 75% are given above the nodes (left side). Bayesian posterior probabilities (BYPP) equal to or greater than 0.95 are given above the nodes (right side). Ex-type strains are in bold and newly generated sequence is in blue bold