Fig. 4.

Supernumerary gametophore formation from multiple apical meristems in the induced PpGRAS12-iOE lines. a Supernumerary gametophores were formed from multiple apical meristems in the PpGRAS12-iOE lines upon PpGRAS12 induction. The red box shows the development of multiple gametophores from apical meristems in the PpGRAS12-iOE lines. Red arrows indicate a single gametophore. Pictures were taken 75 days after the induction; scale bar: 1 mm. b Renewal of induction resulted in the formation of multiple apical meristems and consequently, the formation of multiple gametophores on the top of previous gametophores. The red arrow shows multiple gametophores. Pictures were taken, 12 days after the renewal of induction; scale bar: 1 mm. c Representative WT gametophores grown on standard solid growth medium; scale bar: 100 µm