REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Goat anti-human-IgG	Invitrogen	Cat# A18805; RRID: AB_2535582
Goat anti-human-IgM	Invitrogen	Cat# A18835; RRID: AB_253612
Goat anti-human-IgA	Southern Biotech	Cat# 2050-05; RRID: AB_2687526
Goat anti-mouse IgG	Southern Biotech	Cat# 1033-05; RRID: AB_2737432
Biological samples
Plasma from SJTRC participants	St. Jude Tracking of Viral and Host Factors Associated with COVID-19 study (SJTRC)	NCT04362995
Plasma from FLU09 participants	St. Jude Children’s Research Hospital and the University of Tennessee Health Science Center/Le Bonheur Children’s Hospital	(Allen et al., 2017; Oshansky et al., 2014)
Chemicals, peptides, and recombinant proteins
hCoV-OC43 spike protein	Sino Biological	40607-V08B
hCoV-HKU-1 spike protein	Sino Biological	40606-V08B
hCoV-NL63 spike protein	Sino Biological	40604-V08B
hCoV-229E spike protein	Sino Biological	40605-V08B
SARS-CoV-2 spike protein	Sino Biological	40589-V08B1
His-tag blocking peptide	BioVision	3998BP
Omniblok™ non-fat milk	AmericanBio	AB10109-01000
OPD (o-phenylenediamine dihydrochloride)	Sigma-Aldrich	P8287
SIGMAFAST OPD	Sigma-Aldrich	P9187
Complete Freund’s Adjuvant (including 5mg/mL Mycobacterium tuberculosis)	Chondrex	7023
Critical commercial assays
SARS-CoV-2 Surrogate Virus Neutralization Test Kit	GenScript	L00847-A
Expi293™ Expression Medium	Thermo Fisher Scientific	A1435101
ExpiFectamine 293 transfection kit	Thermo Fisher Scientific	A14524
Deposited data
R script used to run statistical analyses	This paper	https://github.com/SYL16/SJTRC-CCoV
R script used to run statistical analyses	This paper	https://github.com/MacauleyLockeml/St-Jude-Trace-study-SARS-CoV-2
Experimental models: Cell lines
Expi293F cells	Life Technologies	Cat# A14527; RRID:CVCL_D615
Experimental models: Organisms/strains
Mouse: C57BL/6: wildtype	Jackson Laboratories	Cat# 000664; RRID: IMSR_JAX:000664
Recombinant DNA
SARS-CoV-2 nucleocapsid (N) protein plasmid (from Wuhan-Hu-1 isolate)	Florian Krammer	N/A
SARS-CoV-2 spike (S) protein plasmid (from Wuhan-Hu-1 isolate)	Florian Krammer	N/A
SARS-CoV-2 spike protein receptor binding domain (RBD) plasmid (from Wuhan-Hu-1 isolate)	Florian Krammer	N/A
Software and algorithms
R version 4.0.3 and version 3.6.2	The R Core Team	https://www.R-project.org/
R package “TestCor”	(Irene, 2020)	https://cran.r-project.org/web/packages/TestCor/index.html
R package “Psych”	(Revelle, 2021)	https://cran.r-project.org/web/packages/psych/index.html
R package “tidyverse”	(Wickham et al., 2019)	https://cran.r-project.org/web/packages/tidyverse/index.html
R package “vegan”	(Oksanen et al., 2008)	https://cran.r-project.org/web/packages/vegan/index.html
R package “ComplexHeatmap”	(Gu et al., 2016)	http://www.bioconductor.org/packages/devel/bioc/html/ComplexHeatmap.html
R package “Factoextra”	(Kassambara and Mundt, 2017)	https://cran.r-project.org/web/packages/factoextra/index.html
R package “cluster”	(Maechler et al., 2021)	https://cran.r-project.org/web/packages/cluster/index.html
R package “rstatix”	(Kassambara, 2021)	https://cran.r-project.org/web/packages/rstatix/index.html
R package “corrplot”	(Wei et al., 2017)	https://github.com/taiyun/corrplot
R package “circlize”	(Gu et al., 2014)	https://cran.r-project.org/web/packages/circlize/index.html
R package “digest”	(Eddelbuettel et al., 2021)	https://cran.r-project.org/web/packages/digest/index.html
R package “survival”	(Therneau and Lumley, 2015)	https://github.com/therneau/survival
Python version 3.9.8	Python Software Foundation	https://www.python.org/
Seaborn package	(Waskom, 2021)	https://seaborn.pydata.org/