Fig. 7.

IFIT1 regulates pancreatic cancer cell proliferation and migration through the β-catenin pathway. (A-D) Scramble and IFIT1-KD pancreatic cancer cells were pretreated with DMSO or CHIR99021 (6 μM/ml) for 24 h, after which cell proliferation was determined by (A, B) growth curve and (C, D) colony formation assays. (E, F) Cell migration determined by wound healing assay in pretreated Aspc-1 cells. (G) Protein levels of EMT markers and Wnt/β-catenin target genes (β-catenin, cyclin D1, c-Myc and survivin) detected by Western blotting. GAPDH was used as internal control. * p < 0.05, ** p < 0.01, *** p < 0.001