TABLE 2.

Influenza virus reference strains used to evaluate the specificity of the TaqMan-PCR^a

Influenza virus strains	CT ofb:
	Type B-PCR	Type A-PCR	Subtype A H1-PCR	Subtype A H3-PCR
Influenza A (H1N1)
A/PR/8/34	Neg	22	23	Neg
A/Singapore/6/86	Neg	24	24	Neg
A/Taiwan/1/86	Neg	22	23	Neg
A/Texas/36/91	Neg	23	22	Neg
A/Bayern/7/95	Neg	21	22	Neg
Influenza A (H3N2)
A/Leningrad/360/86	Neg	19	Neg	20
A/Sichuan/2/87	Neg	18	Neg	20
A/Shanghai/11/87	Neg	22	Neg	19
A/England/427/88	Neg	20	Neg	21
A/Guizhou/54/89	Neg	19	Neg	19
A/Beijing/353/89	Neg	21	Neg	20
A/Beijing/32/92	Neg	18	Neg	20
A/Shangdong/9/93	Neg	19	Neg	22
A/Johannesburg/33/94	Neg	19	Neg	21
A/Wuhan/353/95	Neg	20	Neg	19
A/Nanchang/933/95	Neg	19	Neg	18
A/Sydney/5/97	Neg	18	Neg	18
Influenza B
B/USSR/100/83	23	Neg	Neg	Neg
B/Ann Arbor/1/86	24	Neg	Neg	Neg
B/Victoria/2/87	22	Neg	Neg	Neg
B/Yamagata/16/88	22	Neg	Neg	Neg
B/Panama/45/90	23	Neg	Neg	Neg
B/Quindao/102/91	20	Neg	Neg	Neg
B/Beijing/184/93	21	Neg	Neg	Neg
B/Harbin/7/94	19	Neg	Neg	Neg
B/Belarus/1/95	22	Neg	Neg	Neg

^aPCR amplification was performed by running 45 cycles with each of the type- and subtype-specific primer/probe systems. 

^bNeg, negative (no increase in fluorescence and, therefore, no amplification was detected during the 45 cycles).