Table 1.

Genes used in this study with PCR primers and process.

Locus	PCR primers	PCR: thermal cycles: (Annealing temp. in bold)	Reference
ITS	ITS1/ITS4	(95 °C: 30 s, 48 °C: 30 s, 72 °C: 1 min) × 35 cycles	White et al. 1990
cal	CAL228F/CAL737R	(95 °C: 15 s, 54 °C: 20 s, 72 °C: 1 min) × 35 cycles	Carbone and Kohn 1999
his3	CYLH3F/H3-1b	(95 °C: 30 s, 57 °C: 30 s, 72 °C: 1 min) × 35 cycles	Crous et al. 2004 Glass and Donaldson 1995
tef1	EF1-728F/EF1-986R	(95 °C: 15 s, 54 °C: 20 s, 72 °C: 1 min) × 35 cycles	Carbone and Kohn 1999
tub2	T1(Bt2a)/Bt2b	(95 °C: 30 s, 55 °C: 30 s, 72 °C: 1 min) × 35 cycles	Glass & Donaldson 1995; O’Donnell and Cigelnik 1997