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. 2021 Dec 6;11:23489. doi: 10.1038/s41598-021-02929-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Activation of TFE3 pathway due flavivirus infection in vertebrate cells. (A) Cells lines Vero-E6 and BHK-21 were transfected with 1 µg of plasmid DNA of transcriptional factor TFE3 (TEF3-Myc). Twenty-four h after transfection, cells were treated with monensin and fixed 24 h after treatment or infected with DENV and ZIKV and fixed 48 hpi. Cells were probed against flavivirus NS1 (green), and Myc (TFE3, red). Nuclei were counter stained with DAPI (blue). Merged fluorescent images of DAPI, red and green channels are shown. The images were analyzed using a LSM 700 confocal microscope with laser sections: 0.45 μm. Images from one out of least 3 independent experiments are shown. (B) Percentage of TFE3 translocated from the cytoplasm to nucleus in both cells lines were measured through images analysis with Zen Blue edition 2.6 software and the results were plotted using GraphPAD Prism version 8.01. *p ≤ 0.05.