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. 2021 Dec 6;11:23489. doi: 10.1038/s41598-021-02929-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Increase expression of genes targeted by the TFR3 pathway in infected cells. BHK-21 cells were infected with DENV and ZIKV using a MOI = 3 and harvested at 24 hpi. Mock infected and monensin treated cells were included as negative and positive controls, respectively. Changes in GOLGA 2 (GM130) and GCP60 gene expression levels were quantified by qRT-PCR using the delta-delta Ct method and GAPDH as housekeeping gene. Results are the mean ± SD of two independent experiments. Differences in mRNA expression levels were analyzed for statistical significance using the ANOVA test. *p ≤ 0.05.