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. 2021 Dec 6;7:376. doi: 10.1038/s41420-021-00771-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Bioinformatics analysis of GSE101123 identified differentially expressed circRNAs including hsa_circRNA_001846 (that is, circRPPH1). The cutoff was set at | log₂(fold change) | > 2 and P < 0.05. b The expression of circRPPH1 was analyzed in BC tissue in GSE101123. c. CircRPPH1 was formed by backsplicing of RPPH1, exon 1. d–e The expression of circRPPH1 and linear RPPH1 were detected after treating total RNA of MDA-MB-231 (d) and MCF-7 (e) cells with RNase R. f The circular structure of circRPPH1 was identified by agarose gel electrophoresis. g The subcellular distribution of circRPPH1 was detected by FISH assay in MDA-MB-231 (up) and MCF-7 (down) cells. DAPI was used for nucleus staining. Blue, DAPI. Red, circRPPH1. h–i The levels of U6 (internal reference for nuclear transcripts), GAPDH (internal reference for cytoplasmic transcripts) and circRPPH1 were determined by RT-qPCR after subcellular fraction. *P < 0.05, **P < 0.01, *** P < 0.001, **** P < 0.0001.