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. 2021 Dec 6;7:376. doi: 10.1038/s41420-021-00771-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Efficiency of LV-circRPPH1 was determined by RT-qPCR. b–c MTT assay was used to compare the proliferation of LV-vector group and LV-circRPPH1 group in MDA-MB-231 (b) and MCF-7 (c) cells. d–e Colony formation assay was conducted to investigate the colony formation ability (d). The number of colonies were counted (e). f–g The migration of MDA-MB-231 cells transfected with LV-vector or LV-circRPPH1 was analyzed by wound-healing assay. Photographs were taken at 0 h and 24 h after scratching (f). Wound closure was analyzed (g). h–i Transwell assay was conducted to analyze the migration of MDA-MB-231 cells after transfecting with LV-vector or LV-circRPPH1. Photographs were taken at 20 h after seeding (h) and cell numbers were counted (i). j–k The protein levels of PCNA in LV-vector group and LV-circRPPH1 group were analyzed by western blotting. ACTIN was employed as internal control. *P < 0.05, **P < 0.01, *** P < 0.001, ****P < 0.0001.