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. 2021 Dec 6;7:376. doi: 10.1038/s41420-021-00771-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a MiR-512-5p level was detected in BC tissues and paired adjacent tissues (N = 40). b The expression of miR-512-5p in BC cells (MDA-MB-231, MCF-7, and BT549) was compared to that in mammary epithelial cells, MCF-10A. c Efficiency of miR-512-5p inhibitor and mimics were determined by RT-qPCR. d–e The proliferation of MDA-MB-231 (d) and MCF-7 (e) cells was detected by MTT assay after transfecting with miR-NC or miR-512-5p inhibitor. f–g Wound-healing assay was conducted to compare the migration of MDA-MB-231 cells in miR-NC group and miR-512-5p inhibitor group. Photographs were taken at 0 h and 24 h after scratching (f). Wound closure was analyzed (g). h–i Colony formation assay was performed in miR-NC group and miR-512-5p inhibitor group in MDA-MB-231 and MCF-7 cells (h). The number of cell colonies were counted (i). j–k The protein levels of PCNA in miR-NC group and miR-512-5p inhibitor group were detected by western blotting. ACTIN was employed as internal control. l–m MTT assay was conducted to compare the proliferation of miR-NC group or miR-512-5p mimics group in MDA-MB-231 (l) and MCF-7 (m) cells. n–o The migration of MDA-MB-231 cells transfected with miR-NC or miR-512-5p mimics was analyzed by wound-healing assay. Photographs were taken at 0 and 24 h after scratching (n). Wound closure was analyzed (o). p–q Colony formation assay was performed to compare the colony formation ability of BC cells in the miR-NC group and miR-512-5p mimics group (p). The number of cell colonies were counted (q). r–s The protein level of PCNA in miR-NC group and miR-512-5p mimics group were analyzed by western blotting. The expression level was normalized to ACTIN. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.