Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 6;7:376. doi: 10.1038/s41420-021-00771-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — a Effect of si-circRPPH1 on the level of STAT1 mRNA was detected by RT-qPCR. b–c Effect of si-circRPPH1 on the protein level of STAT1. ACTIN was employed as internal control. d The transcription level of STAT1 was detected by RT-qPCR after overexpression of circRPPH1. e–f The protein level of STAT1 was analyzed by western blotting after transfecting BC cells with LV-vector or LV circRPPH1. The protein levels were normalized to ACTIN. g–h MTT assay was conducted to investigate whether the inhibitory effect of si-circRPPH1 on BC cell proliferation could be rescued by miR-512-5p inhibitor. i–j The effect of co-transfecting si-circRPPH1 and miR-512-5p inhibitor on colony formation was detected by colony formation assay (i). The cell colony numbers were counted (j). k–p The protein levels of STAT1 and PCNA were presented by western blotting assay in the rescue experiment in both MDA-MB-231 (k–m) and MCF-7 cells (n–p). ACTIN was taken as the internal control of protein levels. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.