REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
R-phycoerythrin labelled goat-anti-human IgG	Dianova	Cat#JIM-109-116-098; RRID: AB_2337678
Biological samples
Pre-pandemic serum samples from healthy adult blood donors (anonymized)	Blutspendedienst des Bayerischen Roten Kreuzes	N/A
Serum specimens from patients with COVID-19 (pseudonymized)	This study	N/A
Chemicals, peptides, and recombinant proteins
HCoV-OC43 spike protein	Sino Biological	Cat#40607-V08B
Critical commercial assays
recomLine SARS-CoV-2 IgG line immunoassay	Mikrogen	Cat#7374
Deposited data
Pseudonymized patient record data and raw data from serum antibody measurements in patients and pre-pandemic donors	This study	Mendeley: https://doi.org/10.17632/stfw4w4vjh.1
Oligonucleotides
Primer: CAG promoter forward CTT CTG GCG TGT GAC CGG	This study	N/A
Primer: CAG promoter reverse CAT GGT GGC CTT TGC CAA	This study	N/A
Primer: T4 foldon forward AAG TGG CCT AGC GGG CGC TTG GTC CCA CGT G	This study	N/A
Primer: T4 foldon reverse AAG ATC TGC TAG CTC GAG TCG C	This study	N/A
Primer: NL63-S1 forward CAT TTT GGC AAA GGC CAC CAT GAA GCT GTT CCT GAT CCT GC	This study	N/A
Primer: NL63-S1 reverse GGA GGA ATT TGC AGG AAT CAG GGA ACC GTC AG	This study	N/A
Primer: NL63-S2 forward CCC TGA TTC CTG CAA ATT CCT CCG ACA ACG GTA TCT	This study	N/A
Primer: NL63-S2 reverse CCA AGC GCC CGC TAG GCC ACT TGA TGT AGT TCT CGA A	This study	N/A
Recombinant DNA
Plasmid: pCAGGS	NovoPro	Cat#V008798
Plasmid: pCAGGS encoding SARS-CoV-2 trimeric spike	Amanat et al. (2020)	N/A
Plasmids: pCAGGS encoding spike S1 domains of SARS-CoV-2, HCoV-229E, HCoV-NL63, HCoV-HKU1 or HCoV-OC43	Becker et al. (2021)	N/A
Plasmids: pRSET2b encoding nucleocapsid proteins of SARS-CoV-2, HCoV-229E, HCoV-NL63, HCoV-HKU1 or HCoV-OC43	Becker et al. (2021)	N/A
Plasmid: pCMV3-C-FLAG encoding HCoV-NL63 spike gene ORF cDNA	Sino Biological	Cat#VG40604-CF
Software and algorithms
recomScan 3.4	Mikrogen	Cat#31006
xPOTENT 4.3	Luminex	Cat# XPON-UPGRD-FM3D
Prism 9.3.0	GraphPad	www.graphpad.com
R version 4.1.1	R Foundation	www.r-project.org
R package tidyverse 1.3.1	Wickham et al. (2019)	cran.r-project.org/package=tidyverse
R package caret 6.0-90	RStudio	cran.r-project.org/package=caret
R package MASS 7.3-54	Venables and Ripley (2002)	cran.r-project.org/package=MASS
Other
Dynablot Plus strip processor	Dynex Technologies	Cat#D7144-P6-E
Flexmap 3D	Luminex	Cat#FLEXMAP-3D
Biomek i7	Beckman	Cat#B87587