Skip to main content
. 2021 Nov 6;297(6):101381. doi: 10.1016/j.jbc.2021.101381

Figure 2.

Figure 2

Intracellular localization of WT and mutant TCF4-B¯ in cultured cells.A, Western blot analysis of HEK293 cells transfected with untagged or E2-tagged WT or mutant TCF4-B¯. TCF4 and GAPDH (loading control) signals were detected with specific antibodies. Molecular mass markers are indicated on the right. B, immunocytochemical analysis of WT or mutant TCF4-B¯ overexpressed in HEK293 cells and of WT or mutant E2-TCF4-B¯ overexpressed in rat hippocampal and cortical primary neurons under basal (CNTR) and depolarized (+KCl) conditions. TCF4, nuclei, and cytoskeleton were visualized with anti-TCF4 or anti-E2 antibody (green, indicated as TCF4-B¯), Hoechst 33342 (blue, indicated as DNA), and anti-tubulin-β antibody (red, indicated as Tub), respectively. The lack of signal in cells not transfected with TCF4 constructs (NT) demonstrates the specificity of anti-TCF4 (HEK293 cells) and anti-E2 (cortical neurons) antibodies. The representative confocal microscopy images are shown. HEK, human embryonic kidney; MMID, Mild-to-moderate intellectual disability; PTHS, Pitt-Hopkins syndrome; RTT-like, Rett-like syndrome; SCZ, Schizophrenia; TCF4, transcription factor 4.