Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 6;12(12):1134. doi: 10.1038/s41419-021-04418-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — A HSC conditioned media (CMs) were used as stimulants for Hep3B proliferation assays. HSC 32 kPa CM promoted Hep3B proliferation as compared to 1 kPa CM. *p < 0.05 by ANOVA, n = 6. B HSC CMs were used as attractants for Hep3B cells in the Transwell assay. CM of HSCs on 32 kPa promoted Hep3B migration as compared with. that of HSCs on 1 kPa. *p < 0.05 by ANOVA, n = 3. C Recombinant FGF2 dose-dependently promoted Hep3B proliferation. *p < 0.05 by ANOVA, n = 4. D CM of HSCs on 32 kPa promoted Hep3B proliferation and this effect of 32 kPa CM was dose-dependently reduced by a neutralizing anti-FGF2 antibody. *p < 0.05 by ANOVA, n = 3. E The effects of HSC CM on Hep3B proliferation were abrogated by E2F3 knockdown in HSCs. *p < 0.05 by ANOVA, n = 5. F CMs were used as attractants in the Transwell assay. CM of control HSCs on 32 kPa promoted Hep3B cell migration as compared to that of control HSCs on 1 kPa, and this effect of 32 kPa CM was abrogated by E2F3 knockdown in HSCs. *p < 0.05 by ANOVA, n = 6. G Serum-starved Hep3B pretreated with a CM at 37 °C for 2 h were resuspended in the CM and Hep3B/CM were then co-injected into SCID mice subcutaneously. Tumor nodules were isolated and quantitated on day 10. *P < 0.05 by ANOVA, n = 5 per group. H Tumor lysates were subjected to WB for α-SMA and Collagen I. *P < 0.05 by ANOVA, n = 3.