Figure 2:

HMGECs were differentiated for two days (see Methods) prior to exposure to PGE₂ or PGF_2α for 3 hours. After incubation, cell viability was assessed with a luminescent ATP-based assay. There were no significant differences between the vehicle control and any of the PGE₂ or PGF_2α concentrations, suggesting that these prostaglandins at physiologic concentrations do not alter cell viability. Further there were no differences between PGE₂ or PGF_2α. Triton-X 100 (1%) was used as a positive control, which differed significantly from all other concentrations (p < 0.001). n = 6 per condition

HMGEC: human meibomian gland epithelial cell

PGE₂: prostaglandin E₂

PGF_2α: prostaglandin F_2α