Figure 3:
(A) HMGECs expressed 39 unique CEs, including 6 oxidized CEs (oxCEs). CE 18:1 was the most abundant, followed by CE 16:0. The chain length varied from 11 carbons to 32 carbons. The double-bond count ranged from 0 to 5. CEs are labeled by carbon number and double-bond count, respectively. When a third number is present, it denotes an oxCE with the corresponding number of oxygenations. n = 28
(B-D) HMGECs expressed 145 TAGs that met the criteria for analysis (see Methods). The carbon count varied from 44 to 72 (B), and the double-bond count varied from 0 to 12 (C). LipidView 1.3 detected the neutral loss of sixteen unique fatty acyl chains (D) from the parent TAGs. FA 18:1 was the most frequently observed. n = 28
HMGEC: human meibomian gland epithelial cell
CE: cholesteryl ester
TAG: triacylglycerol
FA: fatty acid