Fig. 6.

Loss of ASS1 can be partially rescued by exogenous malate. (A) Representative wells from low-density agarose clonogenic tumor formation assays after four weeks are shown. (B) Tumor images were acquired to assess tumor number and tumor size in each cell line. (C) Diameter of crystal violet stained tumor spheroids, measured, and quantified using imagej (> 100 tumors/condition, n = 3). (D) Rescue experiments with supplementation by 1 mM exogenous metabolites (> 100 tumors/condition, n = 3). Error bars represent the standard deviation from the mean.