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. 2021 Dec 6;12:7041. doi: 10.1038/s41467-021-27349-7

Fig. 1. GR activates PD-L1 expression and represses MHC-I expression in human pancreatic cancer cells.

Fig. 1

a, b qPCR analysis of immune inhibitory and immune co-stimulatory genes (a) and genes involved in the MHC-I pathway (b) in SU86.86 cells with or without mifepristone (MIFE, 20 μM, 72 h) treatment. n = 3 technical replicates. c Immunoblotting of PD-L1, MHC-I, and B2M in SU86.86 cells with or without MIFE treatment with the indicated doses for 72 h. d–f Representative flow cytometry plots and quantification (by MFI: mean fluorescence intensity) of cell-surface PD-L1 (d), MHC-I (e), and B2M (f) in SU86.86 cells with or without MIFE (20 μM, 72 h) treatment. n = 3 biological replicates. g qPCR analysis of PD-L1, HLA-A, HLA-B, HLA-C, B2M, and NR3C1 in GR-knockdown SU86.86 cells. n = 3 technical replicates. h, Immunoblotting of PD-L1, MHC-I, B2M, and GR in GR-knockdown SU86.86 cells. i–k Representative flow cytometry plots and quantification of cell-surface PD-L1 (i), MHC-I (j), and B2M (k) in GR-knockdown SU86.86 cells. n = 3 biological replicates. l qPCR analysis of PD-L1, HLA-A, HLA-B, HLA-C, and B2M in GR-knockdown SU86.86 cells, with or without dexamethasone (DEX, 100 nM, 8 h) treatment. n = 3 technical replicates. m Immunoblotting of PD-L1, MHC-I, B2M, p-GR (Ser211), and GR in GR-knockdown SU86.86 cells, with or without dexamethasone (DEX, 100 nM, 8 h) treatment. n Normalized luciferase activity of the reporters containing the indicated human PD-L1 gene promoter regions in GR-knockdown SU86.86 cells, with or without IFNγ (10 ng ml−1, 8 h) treatment. n = 4 wells. o Normalized luciferase activity of the reporters containing the indicated human HLA-B, HLA-C, and B2M gene promoter regions in GR-knockdown SU86.86 cells. n = 4 wells. p Normalized luciferase activity of the reporters containing the human GR-binding elements, PD-L1, HLA-B, HLA-C, and B2M promoter regions in SU86.86 cells with or without DEX (100 nM, 8 h) treatment. n = 4 wells. Statistical significance in a, b, d–g, i–l, and n–p was determined by a two-tailed unpaired t-test. Error bars are s.e.m. Source data are provided as a Source Data file.