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. Author manuscript; available in PMC: 2022 Dec 1.
Published in final edited form as: Behav Neurosci. 2021 Jul 29;135(6):782–803. doi: 10.1037/bne0000482

Figure 2: SD-Nrxn1tm1Sage KO pups emit fewer ultrasonic vocalizations when isolated from the dam.

Figure 2:

SD-Nrxn1tm1Sage Het, KO, and WT siblings from 5 litters were individually removed from the home cage and their ultrasonic calls were recorded for 5 minutes during the first two postnatal weeks. (A–C) Total number of calls emitted in 5 minutes. (D–F) Maximum duration of individual calls emitted. Both male and female pups with biallelic mutations in Nrxn1α emitted fewer calls than their WT and Het littermates when isolated on PN4 (A), PN8 (B) and PN10 (C). The maximum duration of individual calls was shorter in KO females on PN8 (E) and PN10 (F), but not on PN4 (D), compared to WT and Het females. 2Way ANOVA with Tukey’s posthoc within sex: *= p<0.05, ** = p < 0.005, **** = p < 0.0001. (N = 3 WT males, 12 Het males, 6 KO males, 6 WT females, 5 Het females, 4 KO females. 1 KO male died after PN4 and 1 Het male was excluded from the analysis at PN8 because the recording failed).