Figure 4: Juvenile SD-Nrxn1tm1Sage KO males have decreased juvenile rough-and-tumble social play behavior.
(A) The average number of total play events over 4 days. Juvenile KO males have decreased total play (combination of pouncing, pinning, and boxing) relative to WT and Het siblings. (B) The average number of pouncing events over 4 days. KO males pounced less compared to WT males. (C) The number of boxing events over 4 days. Both KO and Het males engaged in fewer boxing events. (D) The average number of pinning events over 4 days. Juvenile KO males did not pin as frequently as their sex-matched WT and Het siblings. 2Way ANOVA with Tukey’s posthoc within sex: *= p<0.05, ** = p < 0.01, *** = p < 0.001, # = p < 0.05 compared to WT and Het females (N = 8 WT males, 8 Het males, 6 KO males, 8 WT females, 8 Het females, 8 KO females).