Fig. 4.
Therapeutic effect of u-ADSC, NASH (4w) u-ADSC, or NASH (12w) u-ADSC administration on a mouse model of NASH. Wild-type u-ADSCs, NASH (4w) u-ADSCs, or NASH (12w) u-ADSCs (7.5 × 105 cells) were administered into the splenic subcapsule of mice with NASH-cirrhosis on week 24 and 26 of AT-HF diet. Liver tissues were sampled on week 28. (A) Hematoxylin and eosin-stained sections and DAB staining of CD11b+, F4/80+, Gr-1+, CD4+, and CD8a cells in livers treated with PBS (control), u-ADSCs, NASH (4w) u-ADSCs, or NASH (12w) u-ADSCs. Bars: 200 μm, ×100 magnification. The DAB+ area, identifying cell infiltration was quantified by using ImageJ (area analyzed = 1.576 mm2, bars: mean ± SD; n = 5 independent biological replicates); white bar: No treatment (PBS); light grey bar: u-ADSCs; dark grey bar: NASH (4w) u-ADSCs; dark grey/hatched bar: NASH (12w) u-ADSCs.