Table 4.

Protein levels at 6 weeks

Protein	WT	n	KI	n	P value
Sham (6 wk)
SERCA2a	1.00±0.28	4	1.25±0.48	3	0.41
PLB	1.00±0.34	4	0.96±0.55	3	0.91
pS16/PLB	1.00±0.34	3	0.57±0.22	3	0.14
RyR2	1.00±0.21	3	0.98±0.20	3	0.91
pS2809/RyR2	1.00±0.23	3	1.36±0.05	2	0.13
PKA [C]	1.00±0.11	3	0.93±0.04	2	0.46
PKARIα dimer/monomer	1.00±0.37	4	0.05±0.02	4	<0.05*
CaMKII	1.00±0.22	3	1.02±0.13	2	0.91
pCaMKII	1.00±0.42	3	1.68±0.56	2	0.21
TAC (6 wk)
SERCA2a	1.00±0.26	4	1.37±0.12	5	<0.05*
PLB	1.00±0.54	4	0.81±0.21	5	0.48
pS16/PLB	1.00±0.16	3	0.91±0.27	4	0.64
RyR2	1.00±0.48	7	0.65±0.25	5	0.17
pS2809/RyR2	1.00±0.10	3	0.98±0.08	3	0.79
PKA [C]	1.00±0.08	3	0.89±0.12	3	0.25
CaMKII	1.00±0.29	4	0.97±0.14	3	0.89
pCaMKII	1.00±0.19	4	1.06±0.26	3	0.74

CaMKII indicates Ca/calmodulin‐dependent protein kinase II; KI, knock‐in; pCaMKII, phosphorylated CaMKII; PKA [C], protein kinase A, catalytic subunit; PKARIα, protein kinase A type I‐α regulatory subunit; PLB, phospholamban; pS16, phosphorylation at serine 16; pS2809, phosphorylation at serine 2809; RyR2, ryanodine receptor 2; SERCA2a, sarcoplasmic reticulum Ca‐ATPase 2a; TAC, transverse aortic constriction; and WT, wild‐type.

^*Indicates significance using Student’s unpaired t‐test.