Figure 3. SDS‐PAGE analysis of immune complexes.

After immunoprecipitate elution in 2×SDS Laemmli buffer, immune complexes were separated on polyacrylamide gels (4%–10%) and stained with silver nitrate. SDS‐PAGE 1 and 2: proteins extracted from human endarterectomy specimens were immunoprecipitated with the P3 and C4 scFv‐Fc (single‐chain fragment variable fused to the crystallizable fragment of immunoglobulin G) (from mammalian cells), respectively. SDS‐PAGE 3: Gal3R (recombinant galectin‐3 protein) was immunoprecipitated with a commercial anti‐GAL3 Ab (anti‐galectin‐3 antibody). MW indicates molecular weight; M, marker; NA, non attributed; PH, proteins from human biopsies.