Fig. 5.

A linear model predicts a younger biological age for worms with downregulation of daf-2. (A) Representative cropped confocal images of body wall muscles from daf-2 RNAi (KD) and EV-treated control (EV) worms at a chronological age of day 14. (B) Blinded, manual scoring of randomized images shows a higher number of normal and lower number of severe muscle phenotypes in KD worms. (C) Boxplot of the predicted age by the full linear model reveals a significant difference between KD and EV worms (Welch two-sample t-test). EV, empty vector, KD, knockdown; RNAi, RNA interference.