Skip to main content
. 2021 Sep 2;10(17):e015868. doi: 10.1161/JAHA.120.015868

Figure 4. 5‐HTR2B (5‐HTR2 subtype B) mRNA and protein levels are regulated by estradiol in the adult mouse heart.

Figure 4

Gonadectomized and adrenalectomized C57BL/6 male and female mice were injected with vehicle (Veh), 1 mg/kg of dexamethasone (Dex), or 10 μg/kg estradiol (estrogen [E2]). RNA and protein were isolated at 6 hours after the treatment. mRNA and representative Western blots of 5‐HTR2B in each treatment group for male (A) and female (B) hearts. mRNA was normalized to cyclophillin B, and protein was normalized to GAPDH. Ordinary 1‐way ANOVA with Dunnett multiple comparisons analysis was used to evaluate differences among group treatments unless otherwise specified. Data represent the mean±SE (n=3–5 mice per group). *P<0.05; ns=not significant.