Table 1.

Main Histological Findings of Female Mice in the Proestrus Cycle (Figure 7) Exposed to Water and Corticosterone Treatments in Response to Ischemia‐Reperfusion Injury Compared With Sham Mice

Figures	Gross appearance	Microscopy	Phenotype (proestrus)
TTC staining
Figure 7Bf	No evidence of infarction	No TTC‐negative areas observed	Sham‐RW
Figure 7Bg	Pallor of infarcted region	TTC‐negative (white) areas indicate MI area	MI/IR‐RW
Figure 7Bh	No evidence of infarction	No TTC‐negative areas observed	Sham‐CW
Figure 7Bi	Prominent infarcted region	Substantial TTC‐negative areas indicating dead tissue	MI/IR‐CW
Hematoxylin‐eosin staining
Figure 7Da,b	No evidence of infarction	Cardiac muscle fibers, nucleus located in the center, blood vessel enclosed in fine endothelium	Sham‐RW
Figure 7De,f	Pallor of infarcted region	Pockets of PMN inflammatory cells infiltration, loss of individual cardiomyocyte boundary	MI/IR‐RW
Figure 7Di,j	Pathological infiltration of inflammatory cells	Pockets of PMN inflammatory cells infiltration	Sham‐CW
Figure 7Dm,n	Prominent infarcted region	Indication of edema, fibrosis, and scar formation; wavy fibers with elongation and narrowing	MI/IR‐CW
Masson trichrome staining
Figure 7Dc,d	No evidence of infarction	Cardiac muscle fibers stained in red, collagenous tissue stained in blue enclosing the blood vessel	Sham‐RW
Figure 7Dg,h	Infarcted region evident	Focal nucleomegaly, acute inflammation	MI/IR‐RW
Figure 7Dk,l	No evidence of infarction	Faint loss of cardiac cell striations	Sham‐CW
Figure 7Do,p	Prominent evidence of infarct	Dense granulation tissue with early scar formation; blue represents region with replacement fibrosis	MI/IR‐CW

All descriptions are mentioned for proestrus; however, similar characteristics were observed with less severity for the estrus‐staged hearts. CW indicates corticosterone water; IR, ischemia reperfusion; MI, myocardial infarction; PMN, polymorphonuclear cells; RW, regular water; and TTC, triphenyltetrazolium chloride.