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. 2021 Sep 2;10(17):e020895. doi: 10.1161/JAHA.121.020895

Figure 4. Hif‐1α (hypoxia‐inducible factor 1‐α), p53, and apoptosis in the myocardial infarction (MI) border zone of cardiomyocyte‐specific Hif‐1α hetero‐knockout (caHetKO) mice on day 5.

Figure 4

A, Expression of Hif‐1α, p53, cPARP (cleaved poly [ADP‐ribose] polymerase), and CC‐3 (cleaved caspase 3) in the MI border zone at day 5 after MI. GAPDH (glyceraldehyde 3‐phosphate dehydrogenase) was used as a loading control (CTL). B, Quantification of Western blots shown in (A); n=4 in each group. Data are shown as a ratio to CTL+sham or CTL+MI mice. C, Representative images of terminal deoxynucleotidyl transferase‐mediated dUTP nick end‐labeling (TUNEL)‐positive cells in CTL and caHetKO mice at day 5 after MI. D, Quantification of TUNEL‐positive cell count per square millimeter in CTL and caHetKO mice; n=5–6 in each group. The arrow indicates TUNEL‐positive nuclei. Bar = 50 μm in 200× images and 20 μm in 400× images. Data are shown as a ratio to the average of CTL+MI group. *P<0.05, **P<0.01 vs CTL, analyzed by 1‐way ANOVA, followed by Tukey post hoc test or Student t test (for Hif‐1α and CC‐3 in B and D). N/A indicates not applicable.