Hybridization of HindIII-digested whole DNA with mef probe. Whole DNA was digested with HindIII and run on a 0.7% agarose gel for 3 h. The mef probe is oligonucleotide probe MF5. Lane 1, 02J1048; lane 2, WA4; lane 3, WA8; lane 4, WA12; lane 5, n011; lane 6, 915. Isolates 02J1048 (mef gene positive), n011 (mef gene positive), and 915 (mef gene negative) are unrelated S. pneumoniae isolates.