FIGURE 3.

Targeting of EphA2 and the Hippo pathway by miR‐125a‐5p. (A) Proteins levels of EphA2, YES1, TAZ, and TEAD2 were detected after transfection with miR‐125a‐5p mimics or inhibitors. (B) EphA2, TAZ, and TEAD2 were detected in subcutaneous tumors using immunohistochemistry. (C) Effects of miR‐125a‐5p on TAZ nuclear translocation were detected with immunofluorescence. (D) Expression levels of TAZ target genes were tested using real‐time qRT‐PCR. Blank, blank control; NC, negative control; miR, miR‐125a‐5p mimics; WT, wild‐type 3′‐UTR plasmid; Mut, mutant 3′‐UTR plasmid; *p < 0.05; **p < 0.01; ***p < 0.001. Bar in B, 50 μm; Bar in C, 100 μm