Table 10.

The primers used for gene expression of tight junction proteins and mucin in broiler chickens on 20 d postchallenge fed different treatment diets.

Genes	Forward primer	Reverse primer	Functions
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH)	CCTCTCTGGCAAAGTCCAAG	GGTCACGCTCCTGGAAGATA	Housekeeping gene
Claudin1	TGGAGGATGACCAGGTGAAGA	CGAGCCACTCTGTTGCCATA	Tight junction protein
Occludin	ACGGCAGCACCTACCTCAA	GGCGAAGAAGCAGATGAG	Tight junction protein
Mucin 2 (Muc2)	ATGCGATGTTAACACAGGACTC	GTGGAGCACAGCAGACTTTG	Mucus secretion to provide protective barrier
Mucin 5ac (Muc5ac)	TGTGGTTGCTATGAGAATGGA	TTGCCATGGTTTGTGCAT	Mucus secretion to provide protective barrier