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. 2021 Dec 4;2(4):100987. doi: 10.1016/j.xpro.2021.100987

Figure 2.

Figure 2

Generation of cerebral organoids

(A) Single-cell suspension.

(B) EBs at day 1 (Day 85 of the protocol), revealing small and normal amounts of dead cells around the EB.

(C) Embedding surface.

(D) Picture of an organoid at day 8, 24 h after Matrigel® embedding showing a budding morphology (arrow), an indication of neuroepithelial formation (Day 90 of the protocol).

(E) An organoid on day 13 (Day 95 of the protocol), with greatly expanded neuroepithelia (arrow).

(F) Cerebral organoids at 40 DIV after EBs embedding (Day 120 of the protocol).