Figure 5.

Controlled cortical impact setup

(A) CCI equipment used in our experiments was Leica™ impact one device for electromagnetic control of the impact attached to a Stoelting™ stereotactic frame.

(B) Skull positioning using the ear bars and teeth bar.

(C) Positioning of the cerebral organoids using sterile spoons into the skull filled with phantom brain mixture.

(D) The ear bar takes direct contact with the phantom brain, allowing the equipment to determine the contact point between the cerebral organoid and impactor tip (zero Z-axis). Impactor and skull ready for impact. The red star identifies the contact sensor, which is clamped to the ear bar.