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MEF culture medium
| Reagent | Final concentration | Amount |
|---|---|---|
| DMEM high glucose medium (Sigma D5796) | N/A | 440 mL |
| FBS (Gibco™ Cat.10082147) | 10% | 50 mL |
| Glutamax (Gibco 25030081) | 1X | 5 mL |
| MEM-NEAA (Gibco 11140-050) | 1X | 5 mL |
| Total | n/a | 500 mL |
Store at 4°C max 2 weeks.
