REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-MAP2 (1:400)	BD Pharmingen	Cat#556320
Anti-Cleaved Caspase-3 (1:500)	Abcam	Cat#ab2302
NSE (1:1000)	ProteinTech	Cat#10149-1-AP
Anti-Tubulin β 3 (TUBB3)/ TUJ1 (1:400)	BioLegend	Cat#801201
Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 594 (1:500)	Invitrogen	Cat#A32744
Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (1:500)	Invitrogen	Cat#A32790
Anti-SOX2 (1:200)	Abcam	Cat#ab97959
Anti-FOXG1 (1:400)	Abcam	Cat#ab18259
Anti-GFAP (1:500)	Abcam	Cat#ab7260
OCT4 (1:200)	Stemgent	Cat#09-0023
Anti-SSEA4 (1:200)	Abcam	Cat#ab16287
Anti-TBR1 (1:200)	EMD Millipore	Cat#AB10554
Chemicals, peptides, and recombinant proteins
DMEM High glucose medium	Sigma-Aldrich	Cat#D5796
DMEM / F12 (1:1) (1X)	Gibco	Cat#11330-032
10% Fetal Bovine Serum	Gibco	Cat#10082147
Glutamax	Gibco	Cat#25030081
MEM-NEAA	Gibco	Cat#11140-050
0.05% Trypsin-EDTA	Gibco	Cat#25300054
1× PBS	HyClone	Cat#SH30256LS
Accutase	Sigma-Aldrich	Cat#SCR005
Matrigel hESC-Qualified Matrix, ∗LDEV-Free	Corning	Cat#354277
ReproTeSR medium	STEMCELL Technologies	Cat#05920
mTeSR1 medium	STEMCELL Technologies	Cat#85850
ReLeSR reagent	STEMCELL Technologies	Cat#05872
HyClone FBS	Cytiva	Cat#SH300070.02
Dimethyl sulphoxide	Sigma-Aldrich	Cat#D2650
10% Neutral Buffered Formalin v/v	Fisher-brand	Cat#032059
Triton X-100	Sigma-Aldrich	Cat#X100-500ML
FluorSave mounting medium	Merck Millipore	Cat#345789
Gelatin from Porcine Skin	Sigma-Aldrich	Cat#G1890-500G
Agarose LE	Thomas Scientific	Cat#C748D75
Xylene	Fisher scientific	Cat#X3S-4
100X Sodium Citrate Buffer	Abcam	Cat#ab64236
30% hydrogen peroxide solution	Sigma-Aldrich	Cat# CAS 7722-84-1
ROCK Inhibitor Y-27632	STEMCELL Technologies	Cat#72302
TrypLE Express Enzyme (1X), no phenol red	Gibco	Cat#12604013
Bovine Serum Albumin, heat shock fraction, suitable for RIA, pH 5.2, ≥96%	Sigma-Aldrich	Cat#A7888-50G
Critical commercial assays
CytoTune-iPS 2.0 Sendai virus (SeV) Reprogramming Kit	Thermo Fisher Scientific	Cat#A16517
StemAb Alkaline Phosphatase Staining Kit II	Stemget	Cat#00-0055
STEMdiff Cerebral Organoid Kit	STEMCELL Technologies	Cat#08570
Experimental models: Cell lines
MRC-G Fibroblast culture	ATCC	Cat#ATCC® CCL171
Experimental models: Organisms/strains
Mouse model	Skull only, strain C56Bl6; Male ∼3 Months old.	Cat#JAX-000664
Other
Cell culture incubator-grade orbital shaker	Thermo Scientific	Cat#88881101
Corning 96-Well, Cell Culture-Treated, Flat-Bottom Microplate	Fisher Scientific	Cat#07-200-627
Corning 96-Well Clear Ultra Low Attachment Microplate	Fisher Scientific	Cat#07-201-680
Corning 24-Well Clear Ultra Low Attachment Microplate	Fisher Scientific	Cat#07-200-602
Corning 6-Well Clear Ultra Low Attachment Microplate	Fisher Scientific	Cat#07-200-601
Nalgene Mr. Frosty isopropanol freezing container	Sigma-Aldrich	Cat#C1562
Neubauer cell counting chamber	Hausser Scientific	Cat#5971R30
Embedding surface	STEMCELL technologies	Cat#08579
0.11 μm PES membrane filter unit	N/A	N/A