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. 2021 Dec 4;2(4):100987. doi: 10.1016/j.xpro.2021.100987

EB seeding medium

Reagent Final concentration Amount
EB formation medium X 15 mL
ROCK inhibitor (10 mM stock) 10 μM final 15 μL
Total n/a 15 mL

Keep at 4°C and use fresh.