Resuspension medium
| Reagent | Final concentration | Amount |
|---|---|---|
| mTeSR™ medium | X | 9 mL |
| ROCK inhibitor (10 mM) | 10 μM∗ | 10 μL |
| Total | n/a | 9 mL |
∗ Consider 1 mL of TrypLE™ existing on 6 well plates from the previous step. Keep at 4°C and use fresh
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