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Site directed mutagenesis and additional activity of PDOKF1. Reactions of PDOKF1 wild-type and variants with phthalate. Purified enzymes (10 μM each) were incubated with 10 μM PDRKF1, 100 μM phthalate in the presence of 1 mM NADH. The reaction product was analyzed by HPLC.
