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Apparent steady-state kinetic parameters of PDOKF1 for different substratesa
| kcat (s−1) | Km (μM) | kcat/Km (mM−1s−1) | Couplingb | |
|---|---|---|---|---|
| Phthalate | 2.1 ± 0.1 | 3.6 ± 0.4 | 583 ± 87 | 1.0 ± 0.4 |
| Terephthalate | 2.0 ± 0.1 | 90 ± 12 | 22 ± 4 | 5.0 ± 0.2 |
Experiments were performed using air-saturated 50 mM Tris, pH 8.0, at 25 °C. The reported parameters are based on oxygen consumption.
Relative amount of NADH oxidized per mol of aromatic substrate hydroxylated.
