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. 2021 Nov 26;20:15330338211060202. doi: 10.1177/15330338211060202

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 6. — Verification of the protein expression levels of hub genes. (A) Immunohistochemistry images of six hub genes were obtained from https://www.proteinatlas.org/ENSG00000117399-CDC20/pathology/lung + cancer#ihc (CDC20), https://www.proteinatlas.org/ENSG00000157456-CCNB2/pathology/lung + cancer#ihc (CCNB2), https://www.proteinatlas.org/ENSG00000134057-CCNB1/pathology/lung + cancer#ihc (CCNB1), https://www.proteinatlas.org/ENSG00000145386-CCNA2/pathology/lung + cancer#ihc (CCNA2), https://www.proteinatlas.org/ENSG00000138160-KIF11/pathology/lung + cancer#ihc (KIF11), and https://www.proteinatlas.org/ENSG00000131747-TOP2A/pathology/lung + cancer#ihc (TOP2A) in THPA. (B) The antibodies used targeted CDC20 (HPA055288 and CAB004525), CCNB2 (CAB009575 and HPA008873), CCNB1 (CAB000115, CAB003804, and HPA061448), CCNA2 (CAB000114), KIF11 (HPA006916, HPA010568, and CAB017617), and TOP2A (HPA006458, HPA026773, and CAB002448). Mann–Whitney U tests were used for assessing significance, with a cut-off P value of .05.

Abbreviation: THPA: The Human Protein Atlas.