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. 2021 Sep 13;10(18):e022139. doi: 10.1161/JAHA.121.022139

Figure 3. Collagen IV–targeted, T‐peptide enhancement of fibrotic mouse hearts by whole‐organ ex vivo imaging.

Figure 3

A, The study design. B, Representative ex vivo Odyssey images demonstrating the biodistribution of sulfo‐cyanine5.5 (Cy5.5) T peptide in perfused, transgenic (Tg) β2‐AR (β‐2‐adrenergic receptor) mice (right column) and nontransgenic (Ntg) controls (left column). Although cardiac uptake increases in Tg animals, the kidney, liver, and lung uptakes are consistently high irrespective of the genotype. C, Quantification of sulfo‐Cy5.5 T‐peptide–mediated cardiac enhancement of fibrotic Tg hearts compared with Ntg controls. The T‐peptide specificity is further observed against reduced accumulation of the control S peptide, but only in Tg mice (ie, at the level of disease). No differences between T and S peptides are observed in Ntg mice at the whole‐organ level. Mean±SEM. N=5 per group. *P<0.05. **P<0.01 by 2‐way ANOVA with Sidak post hoc.