Table 1.

Primer Sequences for Real‐Time PCR

Gene	Forward primer	Reverse primer	Product size (bp)
GAPDH	TGACTCTACCCACGGCAAGTTCAA	ACGACATACTCAGCACCAGCATCA	141
ACE	GTGTTGTGGAACGAATACGC	CCTTCTTTATGATCCGCTTGA	187
AT1‐R	CTCAAGCCTGTCTACGAAAATGAG	GTGAATGGTCCTTTGGTCGT	188
TNF‐α	GCCGATTTGCCACTTCATAC	AAGTAGACCTGCCCGGACTC	209
Interleukin‐6	GCCTATTGAAAATCTGCTCTGG	GGAAGTTGGGGTAGGAAGGA	160
Interleukin‐1β	AGCAACGACAAAATCCCT GT	GAAGACAAACCGCTTTTCCA	209
CD11b	TTACCGGACTGTGTGGACAA	AGTCTCCCACCACCAAAGTG	239
Leptin	CCAAAACCCTCATCAAGACC	GTCCAACTGTTGAAGAATGTCCC	154

ACE indicates angiotensin‐converting enzyme 1; AT1‐R, angiotensin II type 1 receptor; PCR, polymerase chain reaction; and TNF‐α, tumor necrosis factor‐α.